Case Study - Example of the Bioaugmentation process
The time, complexity and cost of a
clean-up is totally dependant on the condition of the water at the point when
you begin treatments.
A typical
example of the remediation for eutrophied ponds - a pond with algae mats,
filamentous algae and/ or high nutrient loading:
Visit 1 -
Analysis
Water quality analysis - water
testing and assessment of, nutrient loading and determination of the biological
diversity of the pond · Setting the criteria for the pond ·
Photograph pond for visual comparison · Calculation and determination of
microbial products required and protocols to be followed · Preparation
of project costing and time line.
Visit 2 - Water
preparation
Application of Nu-Clear. This is a
unique blend of high molecular-weight natural humified peat soil extract and
microbes, which eliminates high concentrations of hydrogen sulphide and other
noxious odors (except mercaptans). In limited applications, Nu-Clear can bind
copper, cadmium and other heavy metals. The all-natural humified soil extract
also acts as a biostimulant to further enhance bacterial activity of
gram-positive bacteria. Slow release, polyphenolic aromatic biopolymer with
cation exchange capacity 350 - 900 mq/100 grams, to promote improved settling.
Reduces clumping of filamentous organisms including some cyanobacteria.
Visit 3 - Begin
Biological Nutrient Removal (BNR)
Application of Eutro-Clear Sludge
Treatment. A synergistic blend of fourteen spore-forming Bacillus strains and
eight gram-negative vegetative strains of bacteria. For
areas of heavy contamination of algae blooms and cyanobacteria in lakes,
ponds and storm water run-off. For heavy nutrient loading from non-point source
contamination from agricultural areas or heavily fertilized lawns and for lakes
that are affected by leachates from septic systems or industrial
areas.
Sludge remediation combats
problems found in sludge at the bottom of ponds, lakes and rivers, particularly
sulfides, mercaptans, ammonia, fatty acids, nitrates and other sources of odor
and pollution. Especially formulated to break down feed wastes and fish manures
and waterfowl. Will break the cycle of sludge build-up, fish suffocation, algae
crashes, over-growth of weeds and cattails.
Visit 4 -
7 -Biological Nutrient Removal (BNR) and sludge reduction
Water testing to determine nutrient
reduction rates. Applications of Eutro-Clear .
After stabilization of the pond, Eutro-Clear Sludge Treatment will begin the
bio-dredging process. Depending upon the initial condition of the water, this
may take several applications over several weeks. Also, at this stage
Ortho-phosphate is assimilated into cellular ATP by the competitive populations
of microorganisms. 50 billion CFU/gram product.
Visit 8 -
Ammonia reduction - Nitrification
Water testing to determine
nutrient reduction rates. Application of Nitro-Clear
. This product provides the essential nitrifying bacteria specifically
selected to bring about nitrification of ammonia nitrogen. Strains of
Nitrobacter and Nitrosomonas, with superior talent for rapid nitrification
under aerobic conditions, at a rate of 500 mg/L/Hr. Particularly where high
build-up of excess nutrients leads to proliferation of excess algae, weeds and
"cat tails". These nitrifiers consume 7.2 ppm of alkalinity for each ppm of
ammonia oxidized to nitrate. Under anaerobic conditions in the soil at the
bottom of the lake/pond the Nitrosomonas will carry out denitrification,
helping to complete the nitrogen cycle. Provided that there is sufficient
oxygen in the system, there is no danger of overdosing, even at 50 times normal
dose.
Visit 9 -
11 - Denitrification
Water testing to determine nutrient
reduction rates. Application of Eutro-Clear .
Reduces turbidity and inhibits sludge build up, improves water clarity and
algae. Eutro-Clear thoroughly cleans the water of residual feed and feces,
while participating in the natural carbon, nitrogen, phosphorus and sulphur
cycles to enhance water quality. It uses a non-pathogenic consortium of
gram-positive spore-forming Bacillus and non-spore forming microbes to
aggressively consume a broad spectrum of pollutants, including surfactants,
food fats and oils, sugars, starch, and nominal amounts of pesticides and
hydrocarbons, while eliminating the formation of hydrogen sulphide and removing
nitrate from the system as nitrogen gas. This step will also address
cyanobacteria and associated toxins.
Visit 12-
Denitrification
Water testing to determine nutrient
reduction rates. Application of denitrifying bacteria. A highly concentrated
dry blend featuring denitrificans, a non-pathogenic chemolithotroph, which
simultaneously oxidizes hydrogen sulfide to sulfate or elemental sulfur and
reduces nitrate to nitrogen gas. Also included are other facultative
heterotrophs and strains of a sulfide oxidizing, facultatively heterotrophic,
gram-positive Bacillus species, to extend the performance and application range
for this product.
Selected species of denitrificans
will denitrify even in the presence of oxygen. All of the strains included in
this formula can utilize carbon dioxide as a carbon source, making this product
especially useful in systems where the carbon source has been exhausted at the
stage denitrification is desired. When targeting excess nitrate in an open
pond: While denitrification can take place in an open pond or stream, the
maximum rate will be 17 mg/L/hr.
Visit 13
- Phosphorous reduction
Water testing to determine nutrient
reduction rates. . If additional phosphorous (SRP) reduction is necessary, it
would be done after the final denitrification. The phosphorous is mineralized
into an insoluble calcium phosphate, which precipitate out of solution, settle
and form a sediment. pH levels are slightly elevated. This precipitate remains
insoluble in pH conditions above 7.0. This is not a "Liming" or "Alum"
treatment but rather a blend of organic and inorganic slow release oxygen
compounds with a product to ortho-phosphate reduction rate of 3:1. As the
phosphorous reduction may affect the pH, any adjustments to the pH would be
done at this stage, using hydrochloric acid.
Bi-Monthly and
Monthly Maintenance
Periodic re-seeding of the microbes
will be necessary to maintain an ecologic balance and to preserve the natural
trophic conditions within the pond. Long term maintenance schedules are
determined by the ability to control point source and non-point source
contamination and associated nutrient loading. Being able to control the source
of the pollution can also assist in increasing the effectiveness of the
remediation and reduce maintenance costs.
